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Expression Profiling by Microarray [ Real-Time - PCR ]

Origen Labs offers gene expression analysis on the complete range of Affymetrix GeneChip® arrays. For a detailed list of GeneChip® arrays available for gene expression, please refer to the Affymetrix website.

 



Our Gene Expression profiling services include:
  • 3’ expression analysis for a wide range of Eukaryotic and Prokaryotic organisms
  • Whole Transcript analysis using the Exon 1.0ST and Gene 1.0ST arrays.This analysis is only available for human, rat and mouse samples
  • Analysis of Formalin Fixed Paraffin Embedded (FFPE) samples – degraded RNA samples can be processed for analysis on either 3’ expression or whole transcript arrays. Origen Labs uses reagents from NuGEN Technologies to process samples derived from FFPE tissues


Our services consist of:

  • Total RNA isolation from various type of samples sources (biopsy tissues, cell lines, blood, FFPE samples)
  • Clean up of RNA samples
  • Quality checks using Agilent Bioanalyzer
  • cDNA and cRNA synthesis
  • cRNA fragmentation
  • Hybridization, staining and washing of arrays
  • Scanning of arrays
  • Data quality checks
  • Study report

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Real Time - PCR [ Express Profiling by Microarray ]
If you are only interested in a few genes, quantitative real time-PCR (qRT-PCR) is a more cost effective method of analysis. qRT-PCR is also used to validate genes of interest identified by microarray studies, such as genes with differential expression in disease tissue or forming signatures indicating response to drug treatment. At Origen Labs, we perform qRT-PCR using TaqMan® technology from Applied Biosystems. We can design primers and probes on request.

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